Fig 4.

Enhancement of antigen-specific antibody production by FomA. (A to D) wt mice immunized with ovalbumin (OVA) (10 μg) alone and in combination with FomA (10 μg/ml) or alum (200 μg). Preimmune sera (Pr) and sera after the final immunization (week 6) were examined by ELISA for quantification of OVA-specific total IgG and IgM levels. (A and B) Anti-OVA IgG (A) and anti-OVA IgM (B), expressed as μg/ml ± SD relative to mice immunized with OVA alone and OVA-FomA. ***, P = 0.0005 and 0.0001 by unpaired t test. (C and D) Anti-OVA IgG (C) and anti-OVA IgM (D), expressed as μg/ml ± SD relative to mice immunized with OVA alone and OVA-alum. ***, P = 0.0001; *, P = 0.04. (E to H) TLR2 KO mice were immunized as described above and OVA-specific IgG (E and G) and IgM (F and H) were quantified by ELISA. ****, P < 0.0001; **, P = 0.009.