Fig 5.

Analysis of IFN-γ release after HPV immunization. PBMCs from 23 healthy volunteers were obtained before (T0) and 15 days after the first (T1, day 15) and third (T2, day 195) quadrivalent HPV (type 6/11/16/18) vaccine doses. Cells were stimulated (empty bars) or not (black bars) with the quadrivalent HPV (type 6/11/16/18) vaccine for 72 h, in the presence or absence of an agonistic anti-ILT2 antibody, and the number of IFN-γ-producing cells was analyzed by an ELISPOT assay. Data correspond to the arithmetic mean and SD. ***, P < 0.001 (two-way analysis of variance); ns, nonsignificant.