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. 2012 Jul;19(7):979–990. doi: 10.1128/CVI.00016-12

Fig 7.

Fig 7

Expression of cytokine mRNAs in the NALTs and cervical lymph nodes. Total RNA was extracted from the NALTs and cervical lymph nodes of mice intranasally treated with PBS, 1 μg of γ-PGA-NPs (NP), 1 μg of SV vaccine (SV), SV vaccine with γ-PGA-NPs (SV+NP), or WV vaccine (WV) from A/PR/8/34 and A/Solomon Islands/3/2006. The mRNA levels of IFN-α in the NALTs (A) and of IFN-β, IFN-γ, IL-4, IL-6, and IL-12 p40 in the cervical lymph nodes (B) were determined by real-time RT-PCR (n = 3). *, P < 0.05 for WV compared to PBS; **, P < 0.05 for SV+NP compared to NP; #, P < 0.05 for A/PR/8/34 SV+NP compared to A/Solomon Islands/3/2006 SV+NP; ##, P < 0.05 for A/PR/8/34 WV compared to A/Solomon Islands/3/2006 WV.