Table 1.
Comparison of neutralization titers obtained with the L2-based assay versus those obtained with the L1-based assaya
| Parameter | Value for virus |
||||
|---|---|---|---|---|---|
| HPV16 | HPV31 | HPV33 | HPV45 | HPV58 | |
| Neutralization titer | |||||
| Nonimmune | <50 | <50 | <50 | <50 | <50 |
| Rabbit anti-L2 L1 assay | 12,150 | 1,350 | 4,050 | 12,150 | 4,050 |
| Rabbit anti-L2 L2 assay | 1.5 × 106 | 1.2 × 105 | 4.6 × 105 | 9.0 × 105 | 2.7 × 105 |
| Fold increase | 120 | 85 | 114 | 74 | 66 |
a
The L1-based neutralization assay was compared to the new L2-based neutralization assay using a rabbit polyclonal serum raised against L2 (11-88×5) as a sample immune serum. Serum from a nonimmune rabbit was used as a control. The fold increase in sensitivity between the two assays is indicated.