Fig 11.

Fluorescence microscopy imaging of FmtA^Δ27 localization in S. aureus. Pictures show localization of GFP-FmtA^Δ27 at the cell septum in S. aureus RN4220 (WT strain) and loss of septal localization in the ΔtarO strain. S. aureus RN4220 cultures and ΔtarO mutant strains were incubated with 1 μM GFP-FmtA^Δ27 at room temperature (RT) for 10 min. Pictures were taken with an epifluorescence microscope after removing unbound GFP-FmtA^Δ27. Fluorescence (GFP), differential interference contrast (DIC), and overlap of GFP and DIC images are shown. Red arrows identify the GFP-FmtA^Δ27 protein in S. aureus cells. Bar, 1 μm. (B) A conceptual model of FmtA location in S. aureus based on GFP-FmtA localization studies.