Fig 4.

The effect of removal of WTAs on binding of FmtA^Δ27 to cell wall. (A) Cell walls (100 μg) of S. aureus RN4220 (WT) and the tarO deletion strain (ΔtarO) were incubated with FmtA^Δ27 at 2.5 μM for 2 h at 4°C and analyzed by 12.5% SDS-PAGE. (B) Cell walls with removed WTA [CW (-TA)] (100 μg) isolated from S. aureus RN4220 were incubated with FmtA^Δ27 (15 μM). Experimental conditions were the same as described for panel A. Samples represent control (C), unbound (U), wash (W), and pellet (P) fractions. The SDS-PAGE gels was stained with Coomassie blue.