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Investigation of the interaction between TAs and protein unrelated to peptidoglycan biosynthesis. Lipoteichoic acids (LTAs; 5 μg) were incubated with bovine serum albumin (BSA; pI 4.6), ovalbumin (pI 4.5 to 4.6), RNaseA (pI 9.63), or carbonic anhydrase (pI 5.88 to 6.55) at 10 μg each in a 15-μl reaction volume. Samples were analyzed by nPAGE/H+. Protein bands were stained with Coomassie blue.
