Fig 1.

Doxycycline synergizes with fluconazole in a dosage-dependent and medium- and carbon source-independent manner. Cells of the C. albicans reference strain SC5314 were spotted onto plates containing different drug combinations. Where indicated, fluconazole (Flu) is present at 10 μg/ml, whereas the concentration of doxycycline (Dox) is expressed in μg/ml. (A) SD plates with 3 days of incubation. (B) YPD plates with 1 day of incubation, RPMI-glucose plates and FBS plates with 2 days of incubation, and SEG plates with 5 days of incubation. (C) SC5314 cells were spotted onto SD plates containing fluconazole and either tetracycline (Tetr), gentamicin, or neomycin. The concentration of the antibiotics is indicated in μg/ml. Plates were incubated for 2 days before being scanned.