Fig 1.

Resistance of genotype 1a and 1b replicon cells selected with asunaprevir (ASV). Selected cell colonies or populations were assayed for sensitivity to asunaprevir and an HCV NS5A replication complex inhibitor (as described in Materials and Methods; NS5A replication complex inhibitor EC₅₀s of 3.3 pM and 1.6 pM against genotype 1a and 1b, respectively). The change in EC₅₀s was plotted as the EC₅₀ of inhibitor for the resistant cells divided by the EC₅₀ for DMSO-treated control cells. The data are mean average fold reduction values obtained from ≥3 independent experiments with asunaprevir (except for genotype 1a 10× colony 2 and all NS5A replication complex inhibitor dose-response values, which were tested only in duplicate). Error bars show standard deviations. GT, genotype; RCI, replication complex inhibitor.