Table 1.

Clonal sequence analysis of NS3 protease domain from genotype 1a replicon cells selected in the presence of asunaprevir

Clone or population	Amino acid replacing wild-type residue
	V36	T40	Q41	V51	R62	L72	D79	T95	V107	I114	R123	P131	I132	G152	R155	R161	D168	I170	V172	N174	L175	G176
ASVc 10× clones
1-1																		T
1-2															K
1-3															K
1-4															K
1-5							E								K					Y
1-6															K
1-7				A											K
1-8															K
1-9								A							K
ASV 10× clones
2-1																	G
2-2										V							G
2-3																	G
2-4																		T
2-5																		T
2-6			E
2-7					K
2-8																		T
2-9																		T
ASV 30× clones
1-1															K
1-2																		T
1-3																		T			P
1-4
1-5																		T
1-6																		T				Q
1-7											G							T
1-8		A													K
1-9															K
1-10															K
ASV 30× populations
1																						Rb
2															K
3												S		V	K	S			E			Rb
4															K
5						Ia,b			Ia						K
6	A												V
7						Ia,b			Ia						K
8																						Rb
9						Ia,b			Ia						K

^aPresent in the wild-type cell line.

^bPublished cell culture-adaptive mutation.

^cASV, asunaprevir.