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. 2012 Jul;56(7):3664–3669. doi: 10.1128/AAC.00266-12

Table 2.

P. multocida and M. haemolytica strains used in this studya

Lane no.b Strain Bacterial species MIC (μg/ml)c
 Presence or absence of the following macrolide resistance gene:
TIP TUL TIL GAM erm(42) msr(E) mph(E)
1 11949 P. multocida >128 >128 >128 64 + + +
2 11952 P. multocida >128 8 >128 8 + − −
3 11953 P. multocida >128 4 128 4 + − −
4 11955 P. multocida >128 4 128 4 + − −
5 11956 P. multocida >128 4 128 4 + − −
6 11957 P. multocida >128 >128 128 128 + + +
7 12591 P. multocida 1 0.5 32 0.5 − − −
8 12593 P. multocida 2 >128 32 64 − + +
9 12594 P. multocida 2 >128 32 64 − + +
10 12595 P. multocida 4 >128 32 64 − + +
11 12596 P. multocida 4 >128 32 64 − + +
12 12599 P. multocida 1 1 32 0.5 − − −
13 12600 P. multocida 1 1 32 0.5 − − −
14 12601 P. multocida 1 0.5 32 0.5 − − −
15 12602 P. multocida 4 >128 32 32 − + +
16 12604 P. multocida 1 0.5 32 0.5 − − −
17 12606 P. multocida >128 >128 >128 128 + + +
18 12608 P. multocida >128 8 >128 16 + − −
19 3358 P. multocida 128 >128 >128 128 + + +
20 3361 P. multocida 2 64 32 32 − + +
21 4407 P. multocida 1 0.5 4 0.5 − − −
22 6052 P. multocida >128 8 >128 8 + − −
23 11933 M. haemolytica 128 8 64 4 + − −
24 11934 M. haemolytica 128 64 128 64 + + +
25 11935 M. haemolytica 0.5 2 4 0.25 − − −
26 11937 M. haemolytica 0.5 2 8 0.5 − − −
27 11938 M. haemolytica 128 128 64 128 + + +
28 12540 M. haemolytica 1 2 32 0.5 − − −
29 12548 M. haemolytica 0.5 128 32 64 − + +
30 12553 M. haemolytica 1 128 32 128 − + +
31 12554 M. haemolytica 1 128 32 128 − + +
32 12557 M. haemolytica 1 128 32 128 − + +
33 12558 M. haemolytica 2 >128 32 128 − + +
34 12565 M. haemolytica 2 2 32 1 − − −
35 12568 M. haemolytica 2 2 32 1 − − −
36 12580 M. haemolytica >128 16 128 8 + − −
37 12581 M. haemolytica >128 16 128 8 + − −
38 12582 M. haemolytica >128 16 128 8 + − −
39 12583 M. haemolytica >128 16 128 8 + − −
40 12584 M. haemolytica >128 128 128 128 + + +
41 12585 M. haemolytica >128 16 128 8 + − −
42 12587 M. haemolytica >128 >128 >128 128 + + +
a

Screening for the erm(42), msr(E), and mph(E) genes was carried out using multiplex PCR in all cases. Strains 3358 and 3361 have been characterized by whole-genome sequencing (3, 4). Class 1 strains with only the erm(42) gene are shown in italic type. Class 2 strains with the msr(E) and mph(E) genes are shown on a gray background. Class 3 strains with the erm(42), msr(E), and mph(E) genes are shown in boldface type on a gray background. Strains containing none of the three macrolide resistance genes are not highlighted by italic or boldface type or by a gray background.

b

The lane numbers are the same as for the gels in Fig. 1.

c

MICs for tildipirosin (TIP), tulathromycin (TUL), tilmicosin (TIL), and gamithromycin (GAM) are shown. The MICs were measured according to CSLI standards (2); E. coli ATCC 25922 (TIP MICs, 8 to 16 μg/ml; TUL MICs, 4 to 16 μg/ml; TIL MICs, 128 to >128 μg/ml; GAM MICs, 8 to 16 μg/ml) and Staphylococcus aureus ATCC 29213 (TIP MICs, 8 to 16 μg/ml; TUL MICs, 4 to 8 μg/ml; TIL MICs, 1 to 2 μg/ml; GAM MICs, 1 to 2 μg/ml) were included as quality control reference strains.