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Kinetic parameters for wild-type and mutant DHFR enzymesa
| Enzyme | DHF Km (μM ± SD) | DHF Km fold loss (SaMut/SaWT) | NAP Km (μM ± SD) | NAP Km fold loss (SaMut/SaWT) | DHF kcat (s−1) | DHF kcat/KM |
|---|---|---|---|---|---|---|
| Sa DHFR | 14.5 ± 0.35 | 1.0 | 38.0 ± 2.3 | 1.0 | 31 | 2.1 |
| Sa (F98Y) DHFR | 7.3 ± 0.40 | 1.0 | 45.1 ± 2.2 | 1.2 | 11.1 | 1.5 |
| Sa (F98I) DHFR | 39.4 ± 3.2 | 2.7 | 58.1 ± 0.45 | 1.5 | 17.5 | 2.3 |
| Sa (H30N) DHFR | 18.9 ± 0.75 | 1.3 | 32.9 ± 2.0 | 1.0 | 13.7 | 1.4 |
Mut, mutant; WT, wild type.
