Fig 1.

Ply released by autolysis cannot reassociate with intact cells. A wild-type (wt) culture was grown overnight and allowed to autolyze. The supernatant was collected, filtered, and added to a Δply culture. Wild-type and Δply cultures without added supernatant from an autolyzed culture were grown as positive and negative controls for the presence or absence of Ply, respectively. All cultures were grown to mid-exponential phase, fractionated into supernatant, cell wall, and protoplast compartments, and assayed for the presence of Ply and CodY by Western blotting. Equal cell equivalents were loaded on the gel. Sup, culture supernatant; CW, cell wall; Prt, protoplast.