Fig 8.

Ply localizes to the cell wall when expressed in Bacillus subtilis. The ply gene was placed under the control of the Pspac* promoter in the amyE locus on the B. subtilis chromosome (strain Ply B.s.). The B. subtilis parent strain, JH642, served as a control. Strains were grown to mid-exponential phase, fractionated into supernatant, cell wall, and protoplast compartments, and assayed for the presence of Ply and CodY by Western blotting. Equal equivalents were loaded on the gel. Sup, culture supernatant; CW, cell wall; Prt, protoplast.