Table 1.
Strains used in this study
| Species and strain | Alternative strain name | Relevant genotype or description | Reference or source |
|---|---|---|---|
| S. pneumoniae | |||
| AC353 | TIGR4 | TIGR4, Smr derivative | 11 |
| AC2394 | acaps TIGR4 | AC353 Δcps4A′-′4L | R. Iyer |
| AC4037 | 2394 Δply::spc | AC2394 Δply::spc, Spcr | This study |
| AC4038 | Ply-HA | AC2934, Ply tagged with HA epitope, Cmr | This study |
| AC4039 | N-1 | AC4038, Ply aa 1-21 removed, Cmr | This study |
| AC4041 | N-4 | AC4038, Ply aa 1-197 removed, Cmr | This study |
| AC4043 | C-2 | AC4038, Ply aa 343-471 removed, Cmr | This study |
| AC4044 | C-3 | AC4038, Ply aa 319-471 removed, Cmr | This study |
| AC4045 | SSRrgB-Ply | AC2394, SS from RrgB fused to ply, Cmr | This study |
| AC4046 | PfoNoSS | AC2394, pfo without SS in ply locus, Cmr | This study |
| AC4047 | AC2394, Ply with Pfo D2 in ply locus, Cmr, HA tag | This study | |
| AC4048 | AC2394, Pfo with Ply D2 in ply locus, Cmr, HA tag | This study | |
| AC4049 | D2 | AC2394, Ply's D2 replacement with GGS linkers, Cmr | This study |
| B. subtilis | |||
| AC4050 | AC4052, Ply under control of Pspac* at amyE locus, Cmr | This study | |
| AC4051 | AC4052, SSRrgB-Ply under control of Pspac* at amyE locus, Cmr | This study | |
| AC4052 | JH642 | trpC2 pheA1 | 13 |
| E. coli | |||
| AC1000 | pAC1000, Cmr | 12 | |
| AC578 | pMmeISpc, Spcr | 19 |