Table 2.
Primers used in this study
| Strain | Primer no. | Primer name | Sequence (5′ → 3′) |
|---|---|---|---|
| AC4037 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP101 | Up R1 | CCGGGCCCCCCCTCGAGGTCTTCTACCTCCTAATAAGTTCCTGGA | |
| KP102 | Spc F2 | CTTATTAGGAGGTAGAAGACCTCGAGGGGGGGCCCGGTACCGAGG | |
| KP103 | Spc R2 | GCATTCTCCTCTCTTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAG | |
| KP104 | Down F3 | CAGATTAAAAAAATTATAAGAGAGGAGAATGCTTGCGACA | |
| KP032 | Down R3 | GCCCATTTACGTCCCATTAG | |
| AC4038 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP081 | Up R1 | TTCTCCCTGATGGGTCATCTTCTACCTCCTAATAAGTTCCTGGA | |
| KP082 | PlyHA F2 | ATTAGGAGGTAGAAGATGACCCATCAGGGAGAAAGTATTGAA | |
| KP034 | Cat R2 | TTCTCCTCTCTTATAAAAGCCAGTCATTAGGCCT | |
| KP031 | Down F3 | GCTTTTATAAGAGAGGAGAATGCTTGCGACA | |
| KP032 | Down R3 | GCCCATTTACGTCCCATTAG | |
| AC4039 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP083 | Up R1 | TACAGAAATATCACTCATCTTCTACCTCCTAATAAGTTCCTGGA | |
| KP084 | PlyCatDown F2 | ATTAGGAGGTAGAAGATGAGTGATATTTCTGTAACAGCTACCAAC | |
| KP032 | PlyCatDown R2 | GCCCATTTACGTCCCATTAG | |
| AC4041 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP089 | Up R1 | GAGATAGACTTGGCGCATCTTCTACCTCCTAATAAGTTCCTGGA | |
| KP090 | PlyHACatDown F2 | ATTAGGAGGTAGAAGATGCGCCAAGTCTATCTCAAGTTGGA | |
| KP032 | PlyHACatDown R2 | GCCCATTTACGTCCCATTAG | |
| AC4043 | KP038 | PlyHA F1 | CAGTCGCCTCTATCCTGGAG |
| KP039 | PlyHA R1 | AGATCCTAAGCATAGTCTGGTACATCGTAGGGGTATCTGTAAGCTGTAACCTTAGTCTC | |
| KP040 | PlyHACatDown F2 | AGCTTACAGATACCCCTACGATGTACCAGACTATGCTTAGGATCTCGAGGTCGACGGTAT | |
| KP032 | PlyHACatDown R2 | GCCCATTTACGTCCCATTAG | |
| AC4044 | KP041 | PlyHA F1 | CAGTCGCCTCTATCCTGGAG |
| KP042 | PlyHA R1 | AGATCCTAAGCATAGTCTGGTACATCGTAGGGGTACGCAACTACATTGTCACGTA | |
| KP043 | PlyHACatDown F2 | TGTAGTTGCGTACCCCTACGATGTACCAGACTATGCTTAGGATCTCGAGGTCGACGGTAT | |
| KP032 | PlyHACatDown R2 | GCCCATTTACGTCCCATTAG | |
| AC4045 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP191 | rrgB SS R1 | TGCTTTATTTGCCATCCCAGCCGCAAAAACTGTTGCAGCTGAAAACAGGCTACTCGCTGTCAGTAATAAGGCAGCAAGCATTGTTAAAAATTTGTTGATTGATTTCATCTTCTACCTCCTAATAAGTTCCTGG | |
| KP190 | rrgB SS F2 | ATTAGGAGGTAGAAGATGAAATCAATCAACAAATTTTTAACAATGCTTGCTGCCTTATTACTGACAGCGAGTAGCCTGTTTTCAGCTGCAACAGTTTTTGCGGCTGGGATGGCAAATAAAGCAGTAAATGACTT | |
| KP032 | Down R2 | GCCCATTTACGTCCCATTAG | |
| AC4046 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP201 | ply::noSSPfo R1 | ACTTTGATTCATCTTCTACCTCCTAATAAGTTCCTGG | |
| KP200 | ply::noSSPfo F2 | AGGAGGTAGAAGATGAATCAAAGTATTGATTCTGGAATATCA | |
| KP199 | ply::pfo R2 | GACCTCGAGATCTTAATTGTAAGTAATACTAGATCCAGGG | |
| KP197 | ply::pfo F3 | ACTTACAATTAAGATCTCGAGGTCGACGGTAT | |
| KP032 | Down R3 | GCCCATTTACGTCCCATTAG | |
| AC4047 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP212 | Ply with Pfo D2 R1 | AAATTTATTACCAGTCTTTTTACCTTCCTTTGGAACAAAACTTTCAATTTTATCTCCATTACTAGCCAAGAGTTTCTTTTTATCGTAATTCA | |
| KP213 | Ply with Pfo D2 F2 | GAAGGTAAAAAGACTGGTAATAAATTTATAGTTGTAGAACGTCAAAAAAGATCCCTTACAACATCACCAAGTGATATTTCTGTAACAGCTACCA | |
| KP214 | Ply with Pfo D2 R2 | AGTTGTTTCTATATAATCTGTTTTATTGTGAACAGCCGCAACTACATTGTCACGTAAA | |
| KP215 | Ply with Pfo D2 F3 | ACAGATTATATAGAAACAACTTCTACAGAGTATTCTAACGGAGATTTACTGCTGG | |
| KP032 | Down R3 | GCCCATTTACGTCCCATTAG | |
| AC4048 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP216 | Pfo with Ply D2 R1 | AAACTCATCGGGTAGCTGATTACCCTCTTTGATGAAACGATTTTCAATACTTTCTCCCTGATGGGTTAAAACTTCATTTCTATTGTAACTTAAGC | |
| KP217 | Pfo with Ply D2 a F2 | ATCAAAGAGGGTAATCAGCTACCCGATGAGTTTGTTGTTATCGAAAGAAAGAAGCGGAGCTTGTCGACAAATACAGTAGATATATCAATAATTGATTCTGTAAATGAC | |
| KP218 | Pfo with Ply D2 a R2 | AGCTGTAACCTTAGTCTCAACATAGTCTGTACTATTTTGAAAGGTAGCAACTGAGTTATCTTTTAAGAAAAC | |
| KP219 | Pfo with Ply D2 b F3 | ACAGACTATGTTGAGACTAAGGTTACAGCTTACAGAAAGGGAAAAATAAACTTAGATCATAGTG | |
| KP229 | Pfo with Ply D2 b R3 | GTACATCGTAGGGGTAATTGTAAGTAATACTAGATCCAGGG | |
| KP228 | Pfo with Ply D2 F4 | GTATTACTTACAATTACCCCTACGATGTACCAGA | |
| KP032 | Down R4 | GCCCATTTACGTCCCATTAG | |
| AC4049 | KP064 | Up F1 | TTCTGGAGTTTCCCGATTTC |
| KP208 | Ply domain 2 R1 | AGAAATATCACTAGAACCACCAGAACCACCCAAGAGTTTCTTTTTATCGTAATTCATAG | |
| KP209 | Ply domain 2 F2 | AAGAAACTCTTGGGTGGTTCTGGTGGTTCTAGTGATATTTCTGTAACAGCTACC | |
| KP210 | Ply domain 2 R2 | AGTAAATCTCCGTTAGAACCACCAGAACCACCAGAACCACCAGAACCACCAGAACCACCCGCAACTACATTGTCACGTAA | |
| KP211 | Ply domain 2 F3 | AATGTAGTTGCGGGTGGTTCTGGTGGTTCTGGTGGTTCTGGTGGTTCTGGTGGTTCTAACGGAGATTTACTGCTGG | |
| KP032 | Down R3 | GCCCATTTACGTCCCATTAG | |
| AC4050 | KP245 | Ply in B.s. F | TAAGGAGGTGTTATATATGGCAAATAAAGCAGTAAATGACTTT |
| KP246 | Ply in B.s. R | CTAGTCATTTTCTACCTTATCTTCTACCTG | |
| AC4051 | KP244 | SS Ply B.s. F | TAAGGAGGTGTTATATATGAAATCAATCAACAAATTTTTAACAATGC |
| KP246 | Ply in B.s. R | CTAGTCATTTTCTACCTTATCTTCTACCTG |