Fig 1.

Induction of lolA expression by LolA(I93C/F140C). (A and B) MY301 cells harboring either pSW77 (A) or pSWIF (B) were grown to the early logarithmic phase of growth (optical density at 600 nm [OD₆₀₀] of 0.2), and the expressions of LolA and LolA(I93C/F140C) were then induced with 0.2% arabinose at time zero (closed circles) or not induced (open circles). Samples were taken periodically to measure the β-galactosidase activity expressed from the lolA-lacZ fusion gene. (C) MY301 (wild-type [W.T.]) and MY304 (cpx::spc) cells harboring either pSW77 or pSWIF were grown as described above, and the expression of the plasmid-encoded LolA protein was induced with 0.2% arabinose. β-Galactosidase activity was measured after 2 h of cultivation.