Fig 2.

Induction of lolA expression by depletion of LolA or LolB. MY425 (A, C, and E) and MY427 (B, D, and F) were grown overnight in LB medium containing 0.2% arabinose and then diluted 100-fold with fresh medium containing 0.2% arabinose (open circles and bars) or 0.2% glucose (closed circles and bars). After 3 h, the cultures were again diluted 100-fold with the same medium and grown for an additional 3 h. At 3 and 6 h after the start of cultivation, samples were taken for measurements of β-galactosidase activity (C and D) and for Western blot analysis (E and F). Cellular growth was monitored by measuring the OD₆₀₀ of the cultures (A and B). Judging from the OD₆₀₀ of the cultures, equivalent amounts of protein were loaded onto each lane for Western blot analysis. The asterisk in panel F indicates an uncharacterized material that seemed to be induced by LolB depletion and to cross-react with the anti-LolB antiserum.