Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli strains | ||
| MC4100 | Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR | 6 |
| MY201 | ara+ revertant of MC4100 | 40 |
| MY301 | MY201 lolA-lacZ | This study |
| MY302 | MY201 lolB-lacZ | This study |
| MY303 | MY201 lolC-lacZ | This study |
| MY304 | MY301 cpxA::spc | This study |
| MY418 | MY201 rprA-lacZ | This study |
| MY419 | MY201 wza-lacZ | This study |
| MY3011 | MY301 rcsF1::mini-Tn10 | This study |
| MY3012 | MY301 rcsC2::miniTn10 | This study |
| MY3018 | MY301 rcsF8::mini-Tn10 | This study |
| MY412 | MY301 ΔrcsA | This study |
| MY413 | MY301 ΔrcsB | This study |
| MY414 | MY301 ΔrcsC | This study |
| MY415 | MY301 ΔrcsD | This study |
| MY416 | MY301 ΔrcsF | This study |
| MY425 | MY301 Pbad-lolA 6×His lolA::Cm | This study |
| MY427 | MY301 Pbad-lolB lolB::Cm | This study |
| Plasmids | ||
| pRS551 | lacZ operon fusion vector; Apr Kmr | 35 |
| pCP20 | Thermoinducible FLP recombinase; Apr Camr | 10 |
| pKD32 | Template plasmid for Cmr cassette amplification | 10 |
| pKD119 | oriR101 repA101(Ts) Pbad-gam-bet-exo Tetr | 10 |
| pBAD18 | Pbad expression vector; Apr | 16 |
| pAM201 | lolA 6×His | 27 |
| pSW77 | Pbad-lolA Cmr | 43 |
| pSWIF | Pbad-lolA(I93C/F140C) Cmr | 43 |
| pMY114 | pBAD18 Pbad-lolA 6×His | This study |
| pMY117 | pBAD18 Pbad-lolB | This study |