Autolysis of E. faecalis strains after addition of penicillin G. Strains are OG1RF (open circles), TX5264 (OG1RF gelE) (filled squares), Liv729 (OG1RF salB) (filled triangles), and Liv1016 (OG1RF gelE salB) (open squares). Error bars represent standard errors of the means from triplicate experiments. Statistically significant differences (P < 0.05) in autolysis were determined at 2 h, 3 h, and 4 h for Liv729 relative to OG1RF and for Liv1016 relative to TX5264, using Student's t test.