Table 1.
E. coli strains and plasmid DNAs used in this study
| E. coli strain or plasmid | Relevant genotype or characteristics | Source, reference, or construction |
|---|---|---|
| E. coli strains | ||
| JW0733 | ΔnadA721∷kan | CGSC (3) |
| SMR5923 | Δ(attλ)∷sulApΩgfp-mut2 | S. Sandler (31) |
| JJC213 | Δrep∷kan | B. Michel (6) |
| RW118 | thr-1 araD139 Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 hisG4(Oc) rpsL31 xyl-5 mtl-1 argE3(Oc) thi-1 sulA211 dnaN+ | R. Woodgate (21) |
| MS100 | RW118 dnaN+ tnaA300∷Tn10 | 42 |
| MS101 | MS100 dnaN159 tnaA300∷Tn10 | 42 |
| MS102 | MS100 dnaN+ tnaA300∷Tn10 lexA3(Ind−) | 42 |
| MS103 | MS100 dnaN159 tnaA300∷Tn10 lexA3(Ind−) | 42 |
| MS104 | MS100 dnaN+ tnaA300∷Tn10 lexA51(Def) | 42 |
| MS105 | MS100 dnaN159 tnaA300∷Tn10 lexA51(Def) | 42 |
| MS116 | MS100 dnaN159 tnaA300∷Tn10 ΔuvrB∷cat Δ(dinB-yafN)∷kan | 42 |
| MS125 | MS100 dnaN159 tnaA300∷Tn10 Δ(dinB-yafN)∷kan | 43 |
| JH103 | MS100 dnaN+ tnaA300∷Tn10 ΔnadA721∷kan | P1(JW0733) × (MS100) |
| JH104 | MS100 dnaN159 tnaA300∷Tn10 ΔnadA721∷kan | P1(JW0733) × (MS101) |
| JH105 | MS100 dnaN+ tnaA300∷Tn10 lexA3(Ind−) ΔnadA721∷kan | P1(JW0733) × (MS102) |
| JH106 | MS100 dnaN+ tnaA300∷Tn10 lexA51(Def) ΔnadA721∷kan | P1(JW0733) × (MS104) |
| JH107 | MS100 dnaN+ tnaA300∷Tn10 Δ(attλ)∷sulApΩgfp-mut2 | P1(SMR5923) × (JH103) |
| JH108 | MS100 dnaN159 tnaA300∷Tn10 Δ(attλ)∷sulApΩgfp-mut2 | P1(SMR5923) × (JH104) |
| JH109 | MS100 dnaN+ tnaA300∷Tn10 lexA3(Ind−) Δ(attλ)∷sulApΩgfp-mut2 | P1(SMR5923) × (JH105) |
| JH110 | MS100: dnaN+ tnaA300∷Tn10 lexA51(Def) Δ(attλ)∷sulApΩgfp-mut2 | P1(SMR5923) × (JH106) |
| RW542 | RW118 lexA51(Def) | R. Woodgate (14) |
| JH111 | MS100 tnaA+ lexA51(Def) Δrep∷kan | P1(JJC213) × (RW542) |
| Plasmids | ||
| pRM200 | Apr; pET11a directing expression of Pol II-D547N(exo−) | This work |
| pWSK29 | Apr; pSC101 origin | 51 |
| pRM100 | pWSK29 bearing polA+ (Pol I) | 30 |
| pRM101 | pWSK29 bearing polB+ (Pol II) | 30 |
| pRM103 | pWSK29 bearing umuD′ and umuC+ (Pol V) | 30 |
| pRM102 | pWSK29 bearing dinB+ (Pol IV) | 30 |
| pJH101 | pWSK29 bearing dinB(V303A-W304G-P305A) (Pol IVR) | 19 |
| pJH102 | pWSK29 bearing dinB(Δ346-351) (Pol IVC) | 19 |
| pJH100 | pWSK29 bearing dinB(D103N) (Pol IV-D103N) | 19 |
| pJH103 | pWSK29 bearing dinB(V303A-W304G-P305A-D103N) (Pol IVR-D103N) | 19 |
| pJH104 | pWSK29 bearing dinB(Δ346-351-D103N) (Pol IVC-D103N) | 19 |
| pJH105 | pWSK29 bearing dinB(D8A) (Pol IV-D8A) | This work |
| pJH107 | pWSK29 bearing dinB(D103A) (Pol IV-D103A) | This work |