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NAD+-dependent ferredoxin reduction with 100% H2 catalyzed by cell extracts of glucose-grown M. thermoacetica. (A) Time course of ferredoxin reduction in the absence and presence of NAD+ (1 mM). (B) Amount of ferredoxin reduced versus the amount of NAD+ added. NADP+ could not substitute for NAD+ in promoting ferredoxin reduction with H2. For assay conditions, see Materials and Methods. The reactions were started with ∼0.2 mg cell extracts with a specific activity of 0.1 unit per mg.
