Fig 2.
Effect of mutations on bacteriophage resistance. Bacteriophages (5 μl of lysates containing ∼109 PFU/ml) were spotted onto lawns of cells in CYE overlay agar. The plates were incubated at 25°C for 24 h to observe lysis. Bacteriophages were spotted in the following order from left to right, as indicated also by the numbers in panel A: top row, ϕCj1, ϕCj13, and ϕCj23; middle row, ϕCj28, ϕCj29, and ϕCj42; bottom row, ϕCj48 and ϕCj54. (A) Wild-type F. johnsoniae CJ1827; (B) CJ1984 (ΔremA); (C) CJ1984 complemented with pRR39, which carries remA; (D) CJ1922 (ΔsprB); (E) CJ1985 (ΔsprB ΔremA); (F) CJ1985 complemented with pRR39.