Fig 5.

Effect of RemA on cell aggregation. Cells of CJ1827 (wild type) and CJ1984 (ΔremA) containing either control plasmid pCP23 (images on the left in panels A and B) or remA-expressing plasmid pRR39 (images on the right in panels A and B) were incubated in EC medium. (A) Observation of cells or cell clumps by phase-contrast microscopy. Bar, 50 μm. (B) Macroscopic observation of cell clumps in culture tubes. (C) Turbidity (OD₆₀₀) of samples taken from the top of the culture tubes. Measurements were obtained from samples taken directly from the tubes and from samples taken after addition of 5 mM d-galactose and incubation for 5 min to cause cell dispersal.