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. 2012 Jul;194(14):3678–3688. doi: 10.1128/JB.00588-12

Fig 7.

Fig 7

Effect of mutations in remC, wza, and wzc on cell aggregation. Cells of CJ1584 [Δ(sprC sprD sprB)], CJ1598 [Δ(sprC sprD sprB) wza::HimarEm1], CJ1602 [Δ(sprC sprD sprB) wzc::HimarEm1], CJ1600 [Δ(sprC sprD sprB) remC::HimarEm1], and CJ1984 (ΔremA) were incubated in EC medium. All strains carried pRR39 (to overexpress RemA) except for CJ1984 (ΔremA), which carried the control plasmid pCP23. To examine the ability of two nonaggregating strains to form aggregates when mixed together, 5 ml of CJ1600 [Δ(sprC sprD sprB) remC::HimarEm1] carrying pRR39 and 5 ml of CJ1984 (ΔremA) carrying pCP23 were mixed in a test tube and incubated for an additional 60 min. (A) Observation of cells or cell clumps by phase-contrast microscopy. Bar, 50 μm. (B) Macroscopic observation of cell clumps in culture tubes. (C) Turbidity (OD600) of samples taken from the top of the culture tubes. Measurements were obtained from samples taken directly from the tubes and from samples taken after addition of 5 mM d-galactose, followed by incubation for 5 min to cause cell dispersal.