Fig 2.

ComN is a late recruit to the division site and colocalizes with DivIVA. (A) Colocalization of FtsZ and ComN. Strain FG925, expressing FtsZ-mCherry (FtsZ-mCh) and GFP-ComN fusions, was grown to mid-log phase (OD₆₀₀ = 0.3 to 0.5) in LB medium supplemented with 0.5% xylose and 100 μM IPTG and imaged as described in Materials and Methods. The arrow marks a Z-ring that is not decorated with ComN. These presumably represent young division complexes. An asterisk marks a ComN ring that is not decorated with FtsZ. These should correspond to septa in the final phase of cytokinesis, when FtsZ has already left the division site. Bar, 5 μm. (B) Colocalization of ComN and DivIVA. Strain FG1254, expressing DivIVA-GFP and ComN-mCherry (ComN-mCh), was grown to mid-log phase (OD₆₀₀ = 0.3 to 0.5) in LB medium supplemented with 0.5% xylose and 100 μM IPTG. The overlay image shows that ComN and DivIVA exhibit essentially perfect colocalization.