Fig 3.

DivIVA is required for ComN localization. The GFP-ComN fusion was introduced into different mutants and localization was assayed in cells grown to mid-log phase. In the case of ftsZ, which is an essential gene, a depletion strain was used and grown for at least five generations in the absence of ftsZ expression before being imaged. For each mutant, a panel corresponding to the GFP-ComN image (gray) and a panel with the overlay between the GFP-ComN channel (green) and membrane channel (red) are shown. ftsZ (FG985), minD (FG932), minJ (FG994), minJ minD (FG1007), divIVA (FG1253), and divIVA minD (FG931) mutant strains are shown as indicated. Scale bar, 5 μm.