Table 4.
Virulence of H5N1 recombinant viruses in mice
| Virus | MLD50 | No. of infected mice/total no. of mice (mean virus titer in sample [log10 EID50/ml] ± SD)b |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lung |
Brain |
Liver |
Spleen |
Kidney |
|||||||
| 3 dpia | 6 dpi | 3 dpi | 6 dpi | 3 dpi | 6 dpi | 3 dpi | 6 dpi | 3 dpi | 6 dpi | ||
| rg325G | >6.7 | 3/3 (3.5 ± 0.3) | 3/3 (2.5 ± 0.9) | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| rg325I | >7.7 | 3/3 (3.3 ± 1.1) | 3/3 (2.8 ± 0.9) | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| rg325* | 6.2 | 3/3 (5.1 ± 0.4)* | 3/3 (3.2 ± 0.7) | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| rg325R | 6.2 | 3/3 (5.4 ± 0.3)** | 3/3 (3.6 ± 1.0) | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| rg325S | 5.5 | 3/3 (5.8 ± 0.7)** | 3/3 (5.0 ± 0.7)** | 0/3 | 2/3 (1.8, 2.1)c | 0/3 | 0/3 | 1/3 (1.8)c | 0/3 | 0/3 | 0/3 |
a
*, the lung titers of corresponding strains were significantly higher than those of rg325G, with a P value of <0.05, as determined by ANOVA; **, the lung titers of the corresponding strains were significantly higher than those of rg325G (P < 0.01 by ANOVA).
b
The lower limits of detection were 101.2 EID50/ml for lung and 100.8 EID50/ml for the other samples.
c
The number(s) in parentheses shows the virus titer in an individual infected mouse.