Fig 6.

Analysis of anti-HSV immune responses in vaccinated (LAW-gB1) and mock-vaccinated (LAW) animals. The timeline of the vaccination schedule and testing for elicited immune responses is as described in Fig. 2. (A) Percentages of anti-HSV-1 (circles)- and anti-HSV-2 (triangles)-neutralizing activity in sera collected at the indicated times and tested at 1:10 (solid symbols) and 1:20 (empty symbols) dilution. The percent neutralization refers to the number of infected cells incubated with the virus alone. Serum samples from naive animals diluted 1:10 showed a neutralization activity of ≤10%. An asterisk indicates a statistically significant increment at P ≤ 0.005. (B) Percentages of gB1-γTL from spleen (circles), iliac lymph nodes (triangles), or bone marrow (squares) as measured by intracellular staining after stimulation with gB1 T epitope (solid symbols) or scrambled peptide (empty symbols). Analysis was performed in animals euthanized at the indicated times. The dotted line indicates the cutoff. The solid line indicates the average value. IFN-γ-secreting CD4⁺ T lymphocytes were below cutoff or undetectable. **, Statistically significant increment at P < 0.001.