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Growth kinetics of ΔUS16, US16stop, and US16-REV viruses in fibroblasts and in endothelial cells. HELFs or HMVECs were infected with the parental RVTRwt, RVTRΔUS16 (clone 5.3), RVTRUS16stop, or RVTRUS16-REV (MOI, 0.1 PFU/cell). The extent of virus replication was then assessed by titrating the infectivity of supernatants of cell suspensions on HELFs using the IE antigen indirect immunoperoxidase staining technique (15). The data shown are the averages of three experiments ± SD.
