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The UL83-encoded pp65 tegument protein does not accumulate in the nuclei of endothelial cells infected with US16-deficient viruses. HMVECs were infected with RVTR, RVTRΔUS16, RVTRUS16stop, or RVTRUS16-REV (MOI, 0.1 PFU/cell). At 8 h p.i., the cells were fixed, permeabilized, and stained with an anti-UL83 (pp65) MAb. Immunofluorescence experiments were repeated three times, and representative results are presented (magnification, ×10).
