Fig 5.

IRF-7and IRF-3 expression is not required for the induction of ISGs in irradiated macrophages. (A and E) IRF-3 and IRF-7 mRNA levels were measured in BL6 macrophages treated as described for Fig. 1. (B and F) IRF-3 and IRF-7 protein levels were measured in mock-treated or irradiated BL6 macrophages at the indicated times postirradiation. Viperin and Mx1 mRNA levels were measured by qRT-PCR in BL6 and IRF-3 (C and D)- or IRF-7 (G and H)-deficient macrophages subjected to mock or gamma irradiation treatments. The relative levels of the indicated transcripts in wt mock-treated samples collected at 1 h postirradiation were set to 1 for each independent experiment. Transcript data were pooled from two to three independent experiments (two replicates per experiment) and are presented as means with the SEM. The results for the Western analyses are representative of three to four independent experiments.