Fig 3.

miR-34a is upregulated during EBV infection of primary B cells and is expressed at elevated levels in EBV- and KSHV-infected lymphoma cell lines. (a) Quantitative RT-PCR of mature miR-34a expression levels from resting B cells through EBV infection and LCL outgrowth. MiR-34a expression is normalized to RNU48 levels under each condition. The data are the average values of experiments with three independent healthy donors. The error bars represent standard errors of the mean. (b) qRT-PCR expression of mature miR-34a levels in resting B cells and B lymphoma cell lines. The plus signs under each cell line indicate whether they are BL derived, EBV infected, KSHV infected, or HIV/AIDS associated. The +(I) under Akata indicates EBV positive but latency I expressing, while all other EBV-positive cells are latency III expressing. The miR-34a expression values plotted are normalized to RNU48 levels in each cell line and expressed relative to CD19⁺ B cell levels. The y axis is in logarithmic scale and split between 1 and 5, while the lower portion is more compact than the upper portion to more easily display the differences between miR-34a-expressing cell lines.