Fig 6.

Inhibition of protein priming by the RT-YMHA mutant in trans. trans-complementation priming reactions were performed in TMnNK using His-RT and His-TP (lanes 1 to 5), and cis-priming was performed using His-MiniRT2 (lanes 6 to 10). RT-YMHA at a 1-, 2-, 4-, or 8-fold molar excess (i.e., 1, 2, 4, or 8 pmol) was added to the trans-complementation (lanes 2 to 5) or cis-priming (lanes 7 to 10) reactions. The priming signals of the RT and TP domains or MiniRT2 in the presence of RT-YMHA are indicated at the bottom as percentages of those in the absence of RT-YMHA (lanes 1 and 6).