Fig 9.

trans-complementation priming reaction using TP and RT domain fragments. (A) trans-complementation priming reactions were performed using the starting GST-RT (RT) plus the starting GST-TP domain (TP, lanes 1 and 16) or the newly constructed TP fragments (lanes 2 to 7), using the starting GST-TP plus the newly constructed RT domain fragments (lanes 8 to 15), or using TP/90–209 plus RT/366–555 (lane 17) in TMnNK (top panel) or TMgNK (bottom panel). The protein priming signals on the TP (top and bottom panels) and RT (top panel) domain fragments are indicated at the bottom of the images, as percentages of the priming signals using the starting TP and RT domain constructs (lanes 1 and 16). The question marks denote that the RT domain signals from these constructs were difficult to quantify due to their comigration with the TP signal (see also panel B). (B) trans-complementation priming reactions were performed using GST-RT/349–575 (lane 1), GST-RT/349–555 (lane 2), or GST-RT/366–555 (lane 3) plus His-TP (lanes 1 to 3) in TMnNK, to show more clearly the priming signals on GST-RT/349–555 and GST-RT/366–555, which were not well resolved from the strong and closely migrating GST-TP signals in panel A (lanes 8 and 12). The protein priming signals on the RT and TP domain fragments are indicated at the bottom of the image, as percentages of the priming signals using the starting (His-) TP and (GST-) RT domain constructs (lane 1).