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Filter binding analyses of the selected aptamers. (a) Filter binding assays were performed with unmodified RNA (2′OH) in the presence of gD proteins (100 and 200 nM) from HSV-1 and HSV-2, and tRNA was used as a nonspecific competitor. (b) The amounts of labeled aptamers (unmodified) retained on the filter in the presence and absence of gD proteins were quantitated with an image analyzer (BAS2000; Fuji Film). The percentages of binding to the gD protein of HSV-1 were plotted.
