Fig 4.
BM and BD compounds inhibit different stages of HIV-1 assembly and maturation. (A) HIV-1NL4-3 titers produced from cells treated with the designated inhibitors (concentration, 50-fold over the EC50) or with a DMSO control. (B) Western blots showing levels of virion-associated CA proteins released into the supernatant (top) or levels of Gag, CA, and MA proteins being produced in cells (center). Cellular tubulin levels are shown as a control for toxicity and loading levels (bottom). Note that virus release was nearly normal in cells treated with the two BM inhibitors but was severely impaired in cells treated with the two BD inhibitors. The experiments were performed twice, and similar results were obtained in both cases, although the absolute viral titers differed in the two experiments.