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Inhibitor resistance mutations can enhance CA-NC assembly in vitro. The assembly of wild-type and mutant CA-NC proteins was assayed as shown in Fig. 1, except that the assembly conditions were more stringent (150 mM NaCl). CA-NC/oligonucleotide ratios were maintained across the 2-fold dilution series, and “1×” represents the concentrations used in the standard assembly assay.
