Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The cytotoxicity of PrAg-U2 to uPAR-expressing tumor cells is blocked by PAI-1. HeLa cells were cultured to 50% confluence, preincubated with serum-free DMEM containing 100 ng/ml of pro-uPA and 1 μg/ml of Glu-plasminogen with or without 2 μg/ml of PAI-1 for 30 min. Then PrAg and PrAg-U2 combined with FP59 (50 ng/ml) were added to the cells and incubated for 6 h. The toxins were removed and replaced with fresh serum-containing DMEM. MTT was added to determine cell viability at 48 h.
