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. Author manuscript; available in PMC: 2012 Jul 10.
Published in final edited form as: Oncogene. 2009 Nov 16;29(6):930–936. doi: 10.1038/onc.2009.368

Figure 4. RNMT is necessary for Cyclin D1 mRNA cap methylation and translation.

Figure 4

IMEC cells were transfected with two independent siRNAs directed against RNMT or Cyclophilin B (negative control), as in figure 1. After 24hrs, cells were lysed and protein and RNA was extracted. a) Whole cell extracts were analysed by Western Blot for expression of RNMT, Cyclin D1 and β-Tubulin. RNA was analysed for relative expression level of b) total Cyclin D1 mRNA and c) Cyclin D1 m7G mRNA. Representative gels are shown. For all charts, average results for three independent experiments are shown and error bars indicate the standard deviation.