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. 2012 Jul 10;6(7):e1719. doi: 10.1371/journal.pntd.0001719

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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BALB/c mice (A, C, E) and C57BL/6 mice (B, D, F) were divided into control (squares) and experimentally bitten groups (circles). Mice in the latter group were exposed to sand fly bites (arrows) in weeks 1–5 and additionally in the week 27 (only BALB/c mice). Levels of specific IgG (A, B); IgG1 (C, D); and IgG2b (E, F) were measured by ELISA at all sampling points. Full circles represent significant difference between control and bitten mice (p<0.05). Data are presented as the means ± standard errors of the means. Two independent studies were done. OD = optical density.