Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jul 10;6(7):e1719. doi: 10.1371/journal.pntd.0001719

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

Sera from BALB/c mice experimentally bitten by P. papatasi (Bitten mice) that were positive for anti-P. papatasi IgG (OD>cut-off: 0.19) were tested by Western blot analysis against (A) P. papatasi salivary gland homogenate, (B) a combination of bacterially-expressed PpSP42 and PpSP28 (Yellow-related protein AF335491 and D7-related protein AF335488, respectively), and (C) a combination of bacterially-expressed PpSP44 and PpSP30 (Yellow-related protein AF335492 and D7-related protein AF335489, respectively). The SDS-PAGE protein profiles of the P. papatasi salivary gland homogenate as well as the recombinant proteins were blotted and stained by Amido Black. Pre-immune sera of BALB/c mice were used as the controls (Neg).