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. 2012 Jul 10;7(7):e40761. doi: 10.1371/journal.pone.0040761

Figure 4. Pro-inflammatory cytokine responses in the colon of C. jejuni infected gnotiotic IL-10−/− mice.

Figure 4

Gnotobiotic IL-10−/− mice were generated by antibiotic treatment and orally infected with either C. jejuni B2 (solid circles) or an apathogenic E. coli strain (crossed circles) derived from the commensal gut microbiota of conventional colonized wildtype mice as described in Methods. Uninfected gnotobiotic IL-10−/− mice served as negative controls (open circles). Concentrations (pg per mg colon) of (A) TNF-α, (B) IFN-γ, and (C) MCP-1 were determined in supernatants of ex vivo colonic cultures at day 7 p.i. by cytometric bead array (CBA). Numbers of analyzed animals are given in parentheses. Means (black bars) and levels of significance (P-values) as compared to the respective group (determined by Student’s t-test) are indicated. Data shown are pooled from at least three independent experiments.