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. 2012 Jul 10;7(7):e40761. doi: 10.1371/journal.pone.0040761

Figure 7. TLR-dependent inflammatory and immune cell responses following C. jejuni infection of gnotobiotic IL-10−/− mice.

Figure 7

Gnotobiotic IL-10−/− (B10 background) as well as TLR-2−/− IL-10−/− and TLR-4−/− IL-10−/− mice were generated by antibiotic treatment and orally infected either with C. jejuni B2 (Cj; solid symbols) or a commensal E. coli strain (Ec; crossed symbols) as described in Methods. The average numbers of apoptotic cells (positive for caspase-3, panel A), T-lymphocytes (positive for CD3, panel B), regulatory T-cells (Tregs, positive for FOXP3, panel C) and B-lymphocytes (positive for B220, panel D) from at least six high power fields (HPF, 400x magnification) per animal were determined microscopically in immunohistochemically stained colon sections at day 7 p.i. Numbers of analyzed animals are given in parentheses. Means (black bars) and levels of significance (P-values) determined by the Student’s t-test are indicated. Data shown are representative for three independent experiments.