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. 2012 Jul 10;7(7):e40761. doi: 10.1371/journal.pone.0040761

Figure 8. TLR-dependent pro-inflammatory cytokine responses in the colon of C. jejuni infected gnotobiotic IL-10−/− mice.

Figure 8

Gnotobiotic IL-10−/− (B10 background) as well as TLR-2−/− IL-10−/− and TLR-4−/− IL-10−/− mice were generated by antibiotic treatment and orally infected either with C. jejuni B2 (Cj; solid symbols) or a commensal E. coli strain (Ec; crossed symbols) as described in Methods. Concentrations (pg per mg colon) of (A) TNF-α, (B) IFN-γ, and (C) IL-6 were determined in supernatants of ex vivo colon cultures at day 7 p.i. by cytometric bead array (CBA). Numbers of analyzed animals are given in parentheses. Means (black bars) and levels of significance (P-values) as compared to the respective group (determined by Student’s t-test) are indicated. Data shown are representative for three independent experiments.