Figure 1. Phred quality scores per position.

Average phred scaled quality scores of two paired-end lanes of 101 bp. The dotted line indicates the cut-off point for further analysis and shows that the minimum average quality score on position 62 is 17 (error prob.: 1/50.12). The different colours indicate the different lanes. One paired-end lane is plotted in dark blue and light blue (different colours for the different read directions) and the other in dark green and light green.