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. 2012 Jul 10;7(7):e38508. doi: 10.1371/journal.pone.0038508

Figure 5. MXD3 structure-function analysis.

Figure 5

(A) Quantification of cell proliferation (as total number of cells relative to control) of DAOY cells transfected with full-length MXD3 or mutated forms as indicated in Figure S2. MXD3-transfected cells showed 50% reduction of proliferation compared with vector-only (pHM6). Transfection of ΔC and E66Q constructs resulted in similar reduction in proliferation as full-length MXD3. Transfection of the ΔSID, Δbasic, E66D and E66N constructs had no effect on proliferation compared to control (ns, not significant; asterisks, p<0.001; n  = 4, one-way ANOVA with Bonferroni’s multiple comparison test). (B) Immunoblot analysis of total protein extracts from DAOY cells transfected with the MXD3 constructs in (B). All constructs used expressed the expected proteins at similar levels. Bottom panel, anti-HA; top panel, anti-β-tubulin used as a load control. Molecular mass markers are indicated on the right.