Figure 5. Frequency distribution (Patch freq.%) of patches containing Ca²⁺activated K⁺(BK) channels drug sensitive and the maximal drug effect (Emax%) at different voltages.

Drug response was evaluated at 2x10⁻⁴M concentration in excised patch experiments at −60 mV and +30 mV (Vm) with 150 mM KCl on both side of the membrane patches, and in the presence of 10⁻⁵M Ca²⁺ ions concentrations in the bath. (A) An higher frequency of finding patches containing BK channels responders to bendroflumethiazide (BFT), acetazolamide (ACTZ), benzimidazolone (NS1619), quercetin (QUERC), ethoxzolamide (ETX), dichlorphenamide (DCP) was found in flexor digitorum brevis (FDB) fibers with respect to soleus (Sol) fibers at both voltages. A low frequency was calculated for resveratrol (RESV) in the same muscle fibers, while methazolamide (MTZ) and hydrochlorthiazide (HCT) were not effective. The data were expressed as means ±E.S. Data (*) were significantly different for P<0.05 in comparison to BFT data. (B) An higher frequency of finding patches containing BK channels responders to ETX and RESV was found in Sol fibers as compared with FDB fibers, but a low frequency of finding BK channels responders to ACTZ, NS1619, ETX, and DCP was found in these fibers. HCT, MTZ and QUERC were not effective in Sol fibers. Data (*) were significantly different for P<0.05 in comparison to ETX and RESV data.