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. 2012 Jul 10;7(7):e40277. doi: 10.1371/journal.pone.0040277

Figure 2. Schematic representation of the recombinant proteins.

Figure 2

(A) Proteins expressed in Pseudomonas aeruginosa. The name of recombinant proteins matched with the represented schematic constructions. S100A8 and S100A9 are in frame with ExoS129 or ExoS54. Chimera proteins are the result of the binding of S100A8 with S100A9 by 6 Glycine amino acid residues. (B) In E. coli, recombinant S100 proteins are fused to GST and produced by a pGEX5x2 plasmid. The GST cleavage is performed by the XA factor. Some S100A9-A8 chimera proteins are truncated (Δ) at the C-terminal part of S100A9-A8, at amino acid 90 (Δ90), 86 (Δ86) or 57 (Δ57) compared to the full length S100A9-A8.