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. Author manuscript; available in PMC: 2013 Jun 27.
Published in final edited form as: J Proteomics. 2012 May 7;75(12):3720–3732. doi: 10.1016/j.jprot.2012.04.035

Figure 3.

Figure 3

Experimental design of ligand treatment using label-swap. Neuroblastoma BE(2)-M17 cells were treated with 10 μM pilocarpine for 5 min (A), with 10 ng/mL EGF for 10 min (B), or with 10 μM muscarine for 1, 5, and 20 min (C). In replicated sets, labeled conditions of treatment and control were swapped in both SILAC doublet and triplet.